Determination of the respiration kinetics for mycelial pellets of Phanerochaete chrysosporium.
Identifieur interne : 000E79 ( Main/Exploration ); précédent : 000E78; suivant : 000E80Determination of the respiration kinetics for mycelial pellets of Phanerochaete chrysosporium.
Auteurs : F C Michel [États-Unis] ; E A Grulke ; C A ReddySource :
- Applied and environmental microbiology [ 0099-2240 ] ; 1992.
Descripteurs français
- KwdFr :
- MESH :
- cytologie : Basidiomycota.
- enzymologie : Basidiomycota.
- métabolisme : Basidiomycota, Oxygène.
- Cinétique.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Oxygen.
- cytology : Basidiomycota.
- enzymology : Basidiomycota.
- metabolism : Basidiomycota.
- Kinetics.
Abstract
In mycelial pellet cultures of the white rot basidiomycete Phanerochaete chrysosporium, low oxygen concentration negatively affects the production of the extracellular lignin peroxidases and manganese peroxidases which are key components of the lignin-degrading system of this organism. To test the hypothesis that oxygen limitation in the pellets is responsible for this effect, oxygen microelectrodes were used to determine oxygen concentration gradients within the mycelial pellets of P. chrysosporium. Pellets were removed from oxygenated cultures, allowed to equilibrate with air, and probed with oxygen microelectrodes. The oxygen profiles were modelled assuming that O2 uptake follows a Michaelis-Menten relationship. The Vmax and Km values for oxygen uptake were 0.76 +/- 0.10 g/m3 of pellet per s and 0.5 +/- 0.3 g/m3, respectively. These kinetic values were used to predict respiration rates in air-flushed cultures, oxygen-flushed cultures, and cultures with large pellets (diameter greater than 6 mm). The predicted respiration rates were independently validated by experimentally measuring the evolution of carbon dioxide from whole cultures.
DOI: 10.1128/AEM.58.5.1740-1745.1992
PubMed: 1622246
PubMed Central: PMC195665
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Determination of the respiration kinetics for mycelial pellets of Phanerochaete chrysosporium.</title><author><name sortKey="Michel, F C" sort="Michel, F C" uniqKey="Michel F" first="F C" last="Michel">F C Michel</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemical Engineering, Michigan State University, East Lansing 48824-1101.</nlm:affiliation><orgName type="university">Université d'État du Michigan</orgName><country>États-Unis</country><placeName><settlement type="city">East Lansing</settlement><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Grulke, E A" sort="Grulke, E A" uniqKey="Grulke E" first="E A" last="Grulke">E A Grulke</name></author><author><name sortKey="Reddy, C A" sort="Reddy, C A" uniqKey="Reddy C" first="C A" last="Reddy">C A Reddy</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1992">1992</date><idno type="RBID">pubmed:1622246</idno><idno type="pmid">1622246</idno><idno type="pmc">PMC195665</idno><idno type="doi">10.1128/AEM.58.5.1740-1745.1992</idno><idno type="wicri:Area/Main/Corpus">000E77</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000E77</idno><idno type="wicri:Area/Main/Curation">000E77</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000E77</idno><idno type="wicri:Area/Main/Exploration">000E77</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Determination of the respiration kinetics for mycelial pellets of Phanerochaete chrysosporium.</title><author><name sortKey="Michel, F C" sort="Michel, F C" uniqKey="Michel F" first="F C" last="Michel">F C Michel</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemical Engineering, Michigan State University, East Lansing 48824-1101.</nlm:affiliation><orgName type="university">Université d'État du Michigan</orgName><country>États-Unis</country><placeName><settlement type="city">East Lansing</settlement><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Grulke, E A" sort="Grulke, E A" uniqKey="Grulke E" first="E A" last="Grulke">E A Grulke</name></author><author><name sortKey="Reddy, C A" sort="Reddy, C A" uniqKey="Reddy C" first="C A" last="Reddy">C A Reddy</name></author></analytic><series><title level="j">Applied and environmental microbiology</title><idno type="ISSN">0099-2240</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (cytology)</term><term>Basidiomycota (enzymology)</term><term>Basidiomycota (metabolism)</term><term>Kinetics (MeSH)</term><term>Oxygen (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Basidiomycota (cytologie)</term><term>Basidiomycota (enzymologie)</term><term>Basidiomycota (métabolisme)</term><term>Cinétique (MeSH)</term><term>Oxygène (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Oxygen</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Basidiomycota</term><term>Oxygène</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Kinetics</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cinétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In mycelial pellet cultures of the white rot basidiomycete Phanerochaete chrysosporium, low oxygen concentration negatively affects the production of the extracellular lignin peroxidases and manganese peroxidases which are key components of the lignin-degrading system of this organism. To test the hypothesis that oxygen limitation in the pellets is responsible for this effect, oxygen microelectrodes were used to determine oxygen concentration gradients within the mycelial pellets of P. chrysosporium. Pellets were removed from oxygenated cultures, allowed to equilibrate with air, and probed with oxygen microelectrodes. The oxygen profiles were modelled assuming that O2 uptake follows a Michaelis-Menten relationship. The Vmax and Km values for oxygen uptake were 0.76 +/- 0.10 g/m3 of pellet per s and 0.5 +/- 0.3 g/m3, respectively. These kinetic values were used to predict respiration rates in air-flushed cultures, oxygen-flushed cultures, and cultures with large pellets (diameter greater than 6 mm). The predicted respiration rates were independently validated by experimentally measuring the evolution of carbon dioxide from whole cultures.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">1622246</PMID><DateCompleted><Year>1992</Year><Month>08</Month><Day>04</Day></DateCompleted><DateRevised><Year>2020</Year><Month>07</Month><Day>24</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0099-2240</ISSN><JournalIssue CitedMedium="Print"><Volume>58</Volume><Issue>5</Issue><PubDate><Year>1992</Year><Month>May</Month></PubDate></JournalIssue><Title>Applied and environmental microbiology</Title><ISOAbbreviation>Appl Environ Microbiol</ISOAbbreviation></Journal><ArticleTitle>Determination of the respiration kinetics for mycelial pellets of Phanerochaete chrysosporium.</ArticleTitle><Pagination><MedlinePgn>1740-5</MedlinePgn></Pagination><Abstract><AbstractText>In mycelial pellet cultures of the white rot basidiomycete Phanerochaete chrysosporium, low oxygen concentration negatively affects the production of the extracellular lignin peroxidases and manganese peroxidases which are key components of the lignin-degrading system of this organism. To test the hypothesis that oxygen limitation in the pellets is responsible for this effect, oxygen microelectrodes were used to determine oxygen concentration gradients within the mycelial pellets of P. chrysosporium. Pellets were removed from oxygenated cultures, allowed to equilibrate with air, and probed with oxygen microelectrodes. The oxygen profiles were modelled assuming that O2 uptake follows a Michaelis-Menten relationship. The Vmax and Km values for oxygen uptake were 0.76 +/- 0.10 g/m3 of pellet per s and 0.5 +/- 0.3 g/m3, respectively. These kinetic values were used to predict respiration rates in air-flushed cultures, oxygen-flushed cultures, and cultures with large pellets (diameter greater than 6 mm). The predicted respiration rates were independently validated by experimentally measuring the evolution of carbon dioxide from whole cultures.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Michel</LastName><ForeName>F C</ForeName><Initials>FC</Initials><Suffix>Jr</Suffix><AffiliationInfo><Affiliation>Department of Chemical Engineering, Michigan State University, East Lansing 48824-1101.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Grulke</LastName><ForeName>E A</ForeName><Initials>EA</Initials></Author><Author ValidYN="Y"><LastName>Reddy</LastName><ForeName>C A</ForeName><Initials>CA</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Appl Environ Microbiol</MedlineTA><NlmUniqueID>7605801</NlmUniqueID><ISSNLinking>0099-2240</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>S88TT14065</RegistryNumber><NameOfSubstance UI="D010100">Oxygen</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010100" MajorTopicYN="N">Oxygen</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1992</Year><Month>5</Month><Day>11</Day><Hour>19</Hour><Minute>15</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>3</Month><Day>28</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1992</Year><Month>5</Month><Day>11</Day><Hour>19</Hour><Minute>15</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">1622246</ArticleId><ArticleId IdType="pmc">PMC195665</ArticleId><ArticleId IdType="doi">10.1128/AEM.58.5.1740-1745.1992</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 1984 Apr;81(8):2280-4</Citation><ArticleIdList><ArticleId IdType="pubmed">16593451</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1983 Mar;45(3):755-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16346241</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Bioeng. 1973 Jan;15(1):27-45</Citation><ArticleIdList><ArticleId IdType="pubmed">4713622</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Microbiol. 1987;41:465-505</Citation><ArticleIdList><ArticleId IdType="pubmed">3318677</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 1981 Mar 31;99(2):373-8</Citation><ArticleIdList><ArticleId IdType="pubmed">7236274</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1991 Aug;57(8):2368-75</Citation><ArticleIdList><ArticleId IdType="pubmed">1768105</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Michigan</li></region><settlement><li>East Lansing</li></settlement><orgName><li>Université d'État du Michigan</li></orgName></list><tree><noCountry><name sortKey="Grulke, E A" sort="Grulke, E A" uniqKey="Grulke E" first="E A" last="Grulke">E A Grulke</name><name sortKey="Reddy, C A" sort="Reddy, C A" uniqKey="Reddy C" first="C A" last="Reddy">C A Reddy</name></noCountry><country name="États-Unis"><region name="Michigan"><name sortKey="Michel, F C" sort="Michel, F C" uniqKey="Michel F" first="F C" last="Michel">F C Michel</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhanerochaeteV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E79 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000E79 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhanerochaeteV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:1622246
|texte= Determination of the respiration kinetics for mycelial pellets of Phanerochaete chrysosporium.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:1622246" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhanerochaeteV1
| This area was generated with Dilib version V0.6.37. |  |